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Table 1 Primer sequences and real-time PCR conditions used for gene expression analysis by qRT-PCR

From: The role of I-FABP as a biomarker of intestinal barrier dysfunction driven by gut microbiota changes in obesity

Gene name

Primer Sequence (5’-3’)

AT

I-FABP

ATGGAAAGGAGCTGATTGCT

59 °C

TTGGCCTCCACTCCTTCATA

TLR4

GATGCCTCTCTTGCATCTGG

60 °C

TCATGAGGGATTTTGCTGAGA

GAPDH

GGCATCGTGGAAGGGCTCATGAC

62 °C

ATGCCAGTGAGCTTCCCGTTCAGC

  1. AT annealing temperature, GAPDH glyceraldehyde-3-phosphate dehydrogenase (housekeeping gene), I-FABP intestinal fatty acid binding protein, TLR4 Toll-like receptor 4