Fig. 3

Effect of RCB on Akt and GSK3β phosphorylation during 3 T3-L1 adipogenesis. a RCB downregulated Akt phosphorylation induced by DMI in 3 T3-L1 adipocytes. The 3 T3-L1 pre-adipocytes were induced to differentiate with DMI media in the absence or presence of RCB for 4 or 7 days, and the phosphorylation levels of Akt were detected with its specific antibody. The results are reported as the means ± SD of three independent experiments. *P < 0.05. **P < 0.01. b Effect of RCB on GSK3β phosphorylation during 3 T3-L1 adipogenesis. The phosphorylation levels of GSK3β were determined using a specific antibody. The results are reported as the means ± SD of three independent experiments. *P < 0.05. c Inhibitory effects of LY294002 on 3 T3-L1 adipocyte differentiation. 3 T3-L1 cells were incubated with or without RCB at a concentration of 50 or 150 μg/ml during differentiation in the presence or absence of LY294002 (10 μM). After differentiation, the TG contents in the 3 T3-L1 adipocytes were determined by a triglyceride assay. The data presented are the mean ± SD from three independent experiments. *P < 0.05. d RCB inhibited DMI-stimulated glucose uptake in 3 T3-L1 adipocytes. Glucose uptake activity in differentiated 3 T3-L1 cells was analyzed by measuring the fluorescence intensity of the cells after incubation with 10 μM 2-NBDG for 2 h. The results are reported as the mean ± SD of three independent experiments. *P < 0.05. **P < 0.01