Fig. 4

Effects of RCB extracts on body weight in HFD-induced obese rats. a Body weight gain. The rats were divided into three groups (n = 10): an ND group given a normal diet (ND), an HFD group fed an HFD, and an HFD + RCB group fed an HFD in addition to treatment with RCB (200 mg/kg BW) orally by gavage once a day for 5 weeks. There were significant differences between the body weights of the HFD and ND (**P < 0.01) and HFD and HFD + RCB groups (*P < 0.05) at the end of the experimental period. b Food intake. The mean daily food consumption was 26.7 g and no significant differences were found in food intake between ND and HFD groups. c Adipose tissue mass. The weight of the epididymal adipose tissue was measured by dividing fatty tissue weight by body weight (fatty tissue/body weight x 100). Values represent the means ± SD; P < 0.05, as shown by ANOVA. Bars labeled with different letters indicate significant differences at according to Duncan’s multiple range test. d The weight of the perirenal adipose tissue was measured by dividing fatty tissue weight by body weight (fatty tissue/body weight x 100). The data presented are the mean ± SD from three independent experiments. Values represent the means ± SD; P < 0.05 as shown by ANOVA. Different letters (a and b) mean that values are significantly different among groups. e Representative hematoxylin and eosin-stained sections of epididymal adipose tissue. The adipocyte sizes from the HFD + RCB group were smaller than those from the HFD only group. The scale bar is 100 μm