Fig. 1

Native PAGE system to separate HDL subclasses. a Diagram depicting gel preparation in our new system. Electrophoresis proceeded in the downward direction. Solutions A–D are described in Methods. TEMED, N,N,N’,N’-tetramethylethylenediamine; APS, ammonium persulfate. b Tube-gel PAGE of human serum. HDL (V) was indicated in red. CV, interassay coefficient of variation; QC, quality control. c Samples were from: a normal neonate, a normal adult, a patient with acute myocardial infarction (AMI), and an adult carrying a CETP mutation. HDL (V) was indicated in red. d Representative densitometric scans