Fig. 3

Elevated glucose infusion rates in αZnT8 KO mice during hypoglycaemic hyperinsulinaemic clamps. Experiments were performed on 12 week old male mice. Animals were fasted for 4 h and rendered hypoglycaemic by infusion of insulin (a). Glucose levels were monitored and glucose infusion rate adjusted accordingly to keep the mice in a hypoglycaemic state at ~60–70 % of the initial value (b). c Blood was collected from tail vein at times 0 and 120 min in tubes filled with EGTA (1.6 mg/ml, Sigma) and aprotinin (250 k-international units/ml; Sigma) for glucagon measurement..n = 4 mice per genotype. Data are mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.005. Other details are given in the Methods section