Fig. 4

Glucagon secretion from αZnT8Tg islets is impaired in vitro. Isolated islets were incubated at 1 mM or 17 mM glucose for 1 h. Released glucagon was measured using an HTRF assay. Transgenic islets secreted significantly less glucagon compared to control when incubated at 1 mM glucose. Data are mean ± SEM, n = 4 separate experiments involving islets from 3 to 4 mice per genotype, *P < 0.05