Fig. 5
Vesicular free Zn2+ assessment in αTC1.9 cells. αTC1.9 cells transfected with an expression vector encoding ZnT8 (αTC1.9 ZnT8) or empty vector (αTC1.9 EV) were incubated with ZinPyr4 (1 μM) for 20 min. before imaging. a Representative fields of view for αTC1.9 EV and αTC1.9 ZnT8 cells. b ZinPyr4 fluorescence intensity was determined on ten fields of view for two acquisitions, with constant light source power and exposure time. c Average traces. Acquisitions were performed under perifusion with imaging buffer and with addition as stated (TPEN 50 μM - pyrithione (5 μM)/Zn2+ (100 μM)). Fluorescence intensity ratio was normalised as F/Fmax