Fig. 6
Cytosolic free Zn2+ concentration measured using eCALWY-4 in αTC1.9 cells. αTC1.9 cells were co-transfected with an eCALWY-4 construct and with an expression vector for ZnT8 (αTC1.9 ZnT8) or empty vector (αTC1.9 EV). a Representative ratiometric images, obtained at steady state (R), under perfusion with the Zn2+ chelator N,N,N’,N’-tetrakis (2 pyridylmethyl) ethylenediamine (TPEN) 50 μM (Rmax) and under perfusion with pyrithione (5 μM)/Zn2+ (100 μM) (Rmin). b Representative traces obtained. Fluorescence intensity ratio was normalised according to the formula: (R-Rmin)/(Rmax-Rmin). c Using R, Rmin and Rmax values, cytosolic free Zn2+ concentrations were calculated for αTC1.9 EV cells (2 experiments - 7 cells) and αTC1.9 ZnT8 cells (2 experiments - 20 cells). d Percentage of cells presenting with a saturated sensor response (ie. cytosolic Zn2+ concentrations > 8 nM)