Fig. 4

The mRNA expression of proliferation and apoptosis genes were measured by use of reverse transcription-quantitative polymerase chain reaction. DDATHF were intraperitoneally (I.P.) injected to the pregnant mice at the optimal dose, 40 mg kg-1 body weight (b/w) on the gestation day 7.5. Embryonic brain tissue samples were collected on the gestation day 11.5 *P < 0.05 NTDs versus control. NTDs, neural tube defects