Fig. 1

HCAL diet increases triglyceride deposition in the maternal liver and alters maternal liver lipid profiles. No effect of metformin. Dams were fed a normal (NORM) or a high calorie (HCAL) diet prior to and during pregnancy ± metformin (MET), as described in the Methods. Maternal liver total triglyceride-associated FA (TG-FA) concentrations were determined on GD19 (a). Saturated fatty acids (SFA, b), monounsaturated FA (MUFA, c), polyunsaturated FA (PUFA, d), and n3 fatty acids (n3FA, e) profiles of the maternal livers were determined on GD19. Data are shown as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001