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Table 1 qPCR primers used to assess gene expression of markers of lipid/fatty acid metabolism

From: The effects of prenatal metformin on obesogenic diet-induced alterations in maternal and fetal fatty acid metabolism

Gene Primer Sequence 5′ _ 3′ Accession numbera
(Probe number)
Chrebp Forward
Reverse 		AATCCCAGCCCCTACACC
CTGGGAGGAGCCAATGTG 		NM_133552.1 (10)
D5d Forward
Reverse 		GAACTCTCTTCTGATTGGAGAGCTA
CCGGAATTCATCAGTGAGC 		AB052085.1 (26)
D6d Forward
Reverse 		AATTTCCAGATTGAGCACCAC
AGTGGGGCAATCTTGTGC 		NM_031344.2 (60)
D9d Forward
Reverse 		GAAGCGAGCAACCGACAG
GGTGGTCGTGTAGGAACTGG 		NM_139192.2 (125)
Elovl2 Forward
Reverse 		AACCTCGGAATCACACTTCTTT
TCCCAGCTGGAGAGAACG 		NM_001109118.1 (22)
Elovl5 Forward
Reverse 		TCGATGCGTCACTCAGTACC
CCTTTGACTCGTGTGTCTCG 		NM_134382.1 (122)
Elovl6 Forward
Reverse 		ATGGATGCAGGAAAACTGGA
GCCCGCTTGTTCATCAGA 		NM_134383.2 (119)
Fasn Forward
Reverse 		GGCCACCTCAGTCCTGTTAT
AGGGTCCAGCTAGAGGGTACA 		NM_017332.1 (6)
Lxrα Forward
Reverse 		CAGGAAGAGATGTCCTTGTGG
TCTTCCACAACTCCGTTGC 		NM_031627.2 (2)
Lxrβ Forward
Reverse 		AGCTCTGCCTACATCGTGGT
GACCCTTCTTCCGCTTGC 		NM_031626.1 (106)
Srebf Forward
Reverse 		ACAAGATTGTGGAGCTCAAGG
TGCGCAAGACAGCAGATTTA 		NM_001276707.1 (77)
  1. Forward and reverse primers for rat genes with GenBank Accession numbers and specific Roche Universal Probe numbers used for assessing mRNA expression in rat tissues by qPCR
  2. aNational Center for Biotechnology Information (NCBI) EntrezGene (http://www.ncbi.nlm.nih.gov/gene)
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Nutrition & Metabolism

ISSN: 1743-7075

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