Fig. 1

Acute effects of free fatty acids on insulin secretion at 5.5 mmol/l glucose. Human islets were incubated at 5.5 mmol/l glucose in the presence or absence of 0.5 mM palmitate (PA), palmitoleate (PAO), stearate (SA), or oleate (OA) for 20 min. a Insulin secretion from statically incubated human islets was expressed as fold secretion at control. b Representative graph of dynamic insulin secretion from perifused islets is shown. Fatty acids were added as indicated. c-e Total, first-phase, and second-phase of fatty acid-stimulated insulin secretion during perifusion was expressed as fold control. Results are means ± SEM of 8 donors. *P < 0.05 vs Control, #P < 0.05 PA vs PAO or SA vs OA