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Fig. 4 | Nutrition & Metabolism

Fig. 4

From: Hepatic adaptations to maintain metabolic homeostasis in response to fasting and refeeding in mice

Fig. 4

Mechanisms that regenerate NAD+ to allow for continued metabolic flux through β-oxidation and the tricarboxylic acid cycle. First, we present a β-OH butyrate dehydrogenase 1 (BDH 1) activity and b BDH1 mRNA expression to understand the potential regeneration of NAD+ as acetoacetate is converted to β-OH butyrate by β-OH butyrate dehydrogenase 1. c BDH2 converts β-OH butyrate to acetoacetate and in turn reduces NAD+ to NADH. d By assessing the relative ratio of BDH1:BDH2 we can see that as fasting duration is extended so is the flux from acetoacetate to β-OH butyrate which will increase the regeneration of NAD+. Finally, we shown that uncoupling protein 2 expression increases with fasting duration (e), leading to decreased synthesis of ATP and decreased hepatic ATP content (f). a,bBars that do not share a common letter differ significantly (P < 0.05; n = 6)

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