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Table 2 Real-time-PCR primer sequences

From: Comparison of maternal isocaloric high carbohydrate and high fat diets on osteogenic and adipogenic genes expression in adolescent mice offspring

Gene

Forward primer

Reverse primer

OPG

5′-GGGCGTTACCTGGAGATCG-3′

3′-CGTTGTCATGTGTTGCATTTCC-5′

RANKL

5′-CAGCATCGCTCTGTTCCTGTA-3′

3′-CTGCGTTTTCATGGAGTCTCA-5′

Ctnnb1

5′-CCTCCCAAGTCCTTTATGAATGG-3′

3′-CCGTCAATATCAGCTACTTGCTCTT-5′

PPARγ2

5′-GCCCTTTGGTGACTTTATGGA-3′

3′-GCAGCAGGTTGTCTTGGATG-5′

LPL

5′- GGGAGTTTGGCTCCAGAGTTT-3′

3′- CGTGTGTGAAATGTCATTGATCC-5′

Lep

5′- GTGGCTTTGGTCCTATCTGTC-3′

3′- CGTGTGTGAAATGTCATTGATCC-5′

AdipoQ

5′- TGTTCCTCTTAATCCTGCCCA-3′

3′- CCAACCTGCACAAGTTCCCTT-5

FASN

5′- AGAGATCCCGAGACGCTTCT-3′

3′-GCTTGGTCCTTTGAAGTCGAAGA-5′

Runx2

5′- CAGCATCCTATCAGTTCCCAA-3′

5′- CAGCGTCAACACCATCATT-3′

GAPDH

5′- GACTTCAACAGCAACTCCCAC-3′

3′- TCCACCACCCTGTTGCTGTA-5′

  1. OPG osteoprotegrin, RANK-L Receptor activator of nuclear factor kappa-B ligand, Ctnnb1 beta-catenin, PPAR γ2 Peroxisome proliferator-activated receptor gamma-2, LPL lipoprotein lipase, Lep leptin, AdipoQ adiponectin, FASN fatty acid synthase, Runx2 Runt-related gene 2, GAPDH glyceraldehyde 3-phosphate dehydrogenase