Skip to main content

Advertisement

Table 2 Real-time-PCR primer sequences

From: Comparison of maternal isocaloric high carbohydrate and high fat diets on osteogenic and adipogenic genes expression in adolescent mice offspring

Gene Forward primer Reverse primer
OPG 5′-GGGCGTTACCTGGAGATCG-3′ 3′-CGTTGTCATGTGTTGCATTTCC-5′
RANKL 5′-CAGCATCGCTCTGTTCCTGTA-3′ 3′-CTGCGTTTTCATGGAGTCTCA-5′
Ctnnb1 5′-CCTCCCAAGTCCTTTATGAATGG-3′ 3′-CCGTCAATATCAGCTACTTGCTCTT-5′
PPARγ2 5′-GCCCTTTGGTGACTTTATGGA-3′ 3′-GCAGCAGGTTGTCTTGGATG-5′
LPL 5′- GGGAGTTTGGCTCCAGAGTTT-3′ 3′- CGTGTGTGAAATGTCATTGATCC-5′
Lep 5′- GTGGCTTTGGTCCTATCTGTC-3′ 3′- CGTGTGTGAAATGTCATTGATCC-5′
AdipoQ 5′- TGTTCCTCTTAATCCTGCCCA-3′ 3′- CCAACCTGCACAAGTTCCCTT-5
FASN 5′- AGAGATCCCGAGACGCTTCT-3′ 3′-GCTTGGTCCTTTGAAGTCGAAGA-5′
Runx2 5′- CAGCATCCTATCAGTTCCCAA-3′ 5′- CAGCGTCAACACCATCATT-3′
GAPDH 5′- GACTTCAACAGCAACTCCCAC-3′ 3′- TCCACCACCCTGTTGCTGTA-5′
  1. OPG osteoprotegrin, RANK-L Receptor activator of nuclear factor kappa-B ligand, Ctnnb1 beta-catenin, PPAR γ2 Peroxisome proliferator-activated receptor gamma-2, LPL lipoprotein lipase, Lep leptin, AdipoQ adiponectin, FASN fatty acid synthase, Runx2 Runt-related gene 2, GAPDH glyceraldehyde 3-phosphate dehydrogenase