Fig. 2

Fold changes from baseline in the expression of genes involved in glycolytic pathway and oxidative metabolism in MNC after ingesting a (a) high-carbohydrate or a (b) high-fat meal between lean and obese subjects. Significant differential responses, as analysed by linear mixed model, were found after high-carbohydrate meal in expression of: PFK (P group = 0.032, and time × group =0.032), PDK4 (P group = 0.002, and time × group =0.002), PPARα (P group =0.024, and time × group =0.014), and UCP3 (P group =0.025, and time × group =0.012). Significant differences at a single time point (120 min or 360 min) between the two groups, as tested by independent sample t-test, have been presented in the figure. Data presented as mean ± SEM. Lean —, ●; obese ----, ○; * P < 0.05