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Fig. 6 | Nutrition & Metabolism

Fig. 6

From: Regulatory landscape of AGE-RAGE-oxidative stress axis and its modulation by PPARγ activation in high fructose diet-induced metabolic syndrome

Fig. 6

Quantitative immunoblotting of HNE-protein adduct levels in plasma and subcellular fractions from kidney and liver. The levels of HNE-protein adducts formed in response to HFD in the absence and presence of RGZ were quantified in plasma and subcellular fractions (Sup and Pellet) from kidney and liver. Representative immunoblots are shown in each panel along with the quantitation. a Levels of plasma HNE; levels of HNE in Sup fractions from (b) kidney and (c) liver; levels of HNE in Pellet fractions from d) kidney and (e) liver. Sup = Triton X-100 soluble fraction; Pellet = Triton X-100 insoluble fraction, which enriches for detergent resistant membranes (e.g., lipid rafts). Data are reported as means ± SEM for three independent experiments performed in duplicate. * P < 0.05; **P < 0.01; *** P < 0.001

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Nutrition & Metabolism

ISSN: 1743-7075

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