Table 1 Metabolic changes in Ldlr-knockout mice after 12 weeks high-cholesterol diet feeding

Metabolic parameter

 Body weight (g)

26.91 (24.38 to 28.04)

27.07 (24.87 to 30.06)

n.s.

 Total plasma cholesterol (mmol/L)

10.55 (8.585 to 11.26)

17.27 (13.64 to 22.93)

p < 0.01

 Hepatic cholesterol (mmol/g)

0.60 (0.25 to 5.59)

12.60 (7.67 to 14.27)

p < 0.01

 Hepatic triglycerides (mmol/g)

1.51 (0.45 to 2.88)

4.05 (3.34 to 4.17)

p < 0.01

Cholesterol balance

(μmol/day/100 g BW)

 Biliary bile acids

32.89 (25.23 to 51.87)

35.21 (22.15 to 61.75)

n.s.

 Fecal bile acid (total) excretion

4.64 (3.74 to 16.55)

15.22 (2.59 to 22.69)

n.s.

 Biliary cholesterol secretion

7.76 (2.44 to 9.31)

34.94 (14.58 to 55.13)

p < 0.001

 Fecal neutral sterols excretion

6.56 (3.97 to 9.47)

448.5 (384.6 to 558.7)

p < 0.001

 Dietary cholesterol

2.93 (2.359 to 2.949)

439.6 (394.4 to 522.8)

p < 0.001

Primary and secondary bile acids in feces

(μmol/day/100 g BW)

 Allo-cholic acid

0.16 (0.09 to 1.28)

0.70 (0.06 to 1.08)

n.s.

 Alpha-muricholic acid

0.86 (0.60 to 2.50)

3.06 (0.21 to 7.25)

n.s.

 Deoxycholic acid

0.97 (0.30 to 3.98)

2.92 (0.12 to 6.33)

n.s.

 Cholic acid

2.86 (0.53 to 5.17)

2.05 (0.06 to 5.98)

n.s.

 Chenodeoxycholic acid

0.29 (0.08 to 0.48)

0.36 (0.04 to 0.59)

n.s.

 Hyodeoxycholic acid

0.10 (0.03 to 0.19)

0.15 (0.05 to 0.40)

n.s.

 Ursodeoxycholic acid

0.10 (0.02 to 0.17)

0.16 (0.05 to 0.50)

n.s.

 Beta-muricholic acid

0.68 (0.17 to 1.69)

2.58 (0.05 to 10.07)

n.s.

 Omega-muricholic acid

1.18 (0.41 to 3.53)

1.74 (0.09 to 3.53)

n.s.