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Table 1 Metabolic changes in Ldlr-knockout mice after 12 weeks high-cholesterol diet feeding

From: High-cholesterol diet does not alter gut microbiota composition in mice

  Control (n = 4–6) High-cholesterol (n = 5–8) Significance
Metabolic parameter    
 Body weight (g) 26.91 (24.38 to 28.04) 27.07 (24.87 to 30.06) n.s.
 Total plasma cholesterol (mmol/L) 10.55 (8.585 to 11.26) 17.27 (13.64 to 22.93) p < 0.01
 Hepatic cholesterol (mmol/g) 0.60 (0.25 to 5.59) 12.60 (7.67 to 14.27) p < 0.01
 Hepatic triglycerides (mmol/g) 1.51 (0.45 to 2.88) 4.05 (3.34 to 4.17) p < 0.01
Cholesterol balance
(μmol/day/100 g BW)
   
 Biliary bile acids 32.89 (25.23 to 51.87) 35.21 (22.15 to 61.75) n.s.
 Fecal bile acid (total) excretion 4.64 (3.74 to 16.55) 15.22 (2.59 to 22.69) n.s.
 Biliary cholesterol secretion 7.76 (2.44 to 9.31) 34.94 (14.58 to 55.13) p < 0.001
 Fecal neutral sterols excretion 6.56 (3.97 to 9.47) 448.5 (384.6 to 558.7) p < 0.001
 Dietary cholesterol 2.93 (2.359 to 2.949) 439.6 (394.4 to 522.8) p < 0.001
Primary and secondary bile acids in feces
(μmol/day/100 g BW)
   
 Allo-cholic acid 0.16 (0.09 to 1.28) 0.70 (0.06 to 1.08) n.s.
 Alpha-muricholic acid 0.86 (0.60 to 2.50) 3.06 (0.21 to 7.25) n.s.
 Deoxycholic acid 0.97 (0.30 to 3.98) 2.92 (0.12 to 6.33) n.s.
 Cholic acid 2.86 (0.53 to 5.17) 2.05 (0.06 to 5.98) n.s.
 Chenodeoxycholic acid 0.29 (0.08 to 0.48) 0.36 (0.04 to 0.59) n.s.
 Hyodeoxycholic acid 0.10 (0.03 to 0.19) 0.15 (0.05 to 0.40) n.s.
 Ursodeoxycholic acid 0.10 (0.02 to 0.17) 0.16 (0.05 to 0.50) n.s.
 Beta-muricholic acid 0.68 (0.17 to 1.69) 2.58 (0.05 to 10.07) n.s.
 Omega-muricholic acid 1.18 (0.41 to 3.53) 1.74 (0.09 to 3.53) n.s.
  1. Data are given as median and range. Statistical significance was tested with Mann–Whitney U-test or two-way ANOVA post-hoc Bonferoni test