Fig. 1

Analysis of klf-2 expression with GFP reporter during C. elegans development. Lines of transgenic C. elegans carrying the klf-2::gfp reporter gene was created as described in the “Methods”. a the structure of klf-2 gene; b the klf-2::gfp reporter gene construct; c the fluorescence microscopic images of klf-2 expression: a) The gfp expression was observed along the length of early larval (L1-L2) intestine (solid line); b) GFP is present along the length of the intestine of young adult hermaphrodites (solid line); and c) GFP is present along the length of intestine (one long solid line) in egg-laying hermaphrodites. GFP expression is not present in gonads (short solid lines) and vulva (v). Transgenic C. elegans worms were observed and photographed using Axioskop 2 plus fluorescent microscope (Zeiss, Germany) with appropriate filter sets for GFP (magnification: 200X)