Fig. 4

The effects of dietary salts on klf-2 and −3 expression in wild type and mutant C. elegans. Equal amounts of C. elegans were harvested after treatments. The mRNA purification, cDNA synthesis, qRT-PCR, and the data analysis were performed as described in the “Methods”. The mRNA levels of klf-2 and −3 were normalized against ama-1 as the internal control. The linear fold-change was calculated to compare the difference between the treatments and the control (worms cultured on the regular media-NGM). a klf-2 expression in WT (left panel) and klf-3 mutant (ok1975) (right panel); b klf-3 expression in WT (left panel) and klf-2 mutant (ok1043) (right panel). Each treatment was repeated three times, and the means with standard errors were presented in all panels. * p < 0.05 and ** p < 0.01, indicating significant difference as compared to the control (NGM)