Fig. 4

The combined effect of maternal high-fat diet and variable stress on GSIS (a), pancreatic GLUT2 mRNA expression (b) and protein amount (c) in the young adult offspring. Insulin secretion from the isolated islets in response to 5.6 and 16.7 mM glucose is shown (n = 8 per group) (a). Representative bands for the end product of RT-PCR are shown above the fold change (n = 3 per group) (b). Representative bands are shown above the respective densitometric values (n = 3 per group) (c). Each column denotes mean ± SEM; NC: Normal diet Control, NS: Normal diet Stress, HFC: High-fat diet Control, HFS: High-fat diet Stress, ^** P < 0.01, ^*** P < 0.001 vs. NC, ^†† P < 0.01, ^††† P < 0.001 vs. NS, ^$$$ P < 0.001 vs. HFC, ^ωω P<0.01, ^ωωω P < 0.001 vs. 5.6 mM of the same group