Essential amino acid ingestion alters expression of genes associated with amino acid sensing, transport, and mTORC1 regulation in human skeletal muscle

Table 2 Primer details (SESN2, B2M and GAPDH)

Gene	Sequence (5′- > 3′)	Length	Start	Stop	Tm	GC%	Length	Accession #
SESTRIN2	FWD GCCACTCAGAGAAGGTCCAC	20	1739	1758	60.04	60		NM_001199933.1
SESTRIN2	REV: GAGTCAGGTCATGTAGCGGG	20	1842	1823	59.9	60	104	Exon 1752/1753 fwd
B2M	FWD: CCAAAGATTCAGGTTTACTCAC	22			52.5	40.9		NM_00404
B2M	REV: TCAACTTCAATGTCGGATGG	20			52.8	45	101
GAPDH	FWD: AGGTGAAGGTCGGAGTCAAC	20			56.3	55		J02642
GAPDH	REV: GCTCCTGGAAGATGGTGATG	20			55	55	231

ISSN: 1743-7075