Skip to main content
Fig. 6 | Nutrition & Metabolism

Fig. 6

From: eIF2α phosphorylation is required to prevent hepatocyte death and liver fibrosis in mice challenged with a high fructose diet

Fig. 6

eIF2α phosphorylation is required to prevent ROS accumulation and oxidative damage in liver tissues of HFrD-fed mice. a Fluorescence microscope observation of frozen liver sections stained with dihydroethidium (DHE) from 13-month-old Cont. and A/A Hep mice fed an HFrD for 5 wks, reflecting ROS levels. Representative images are shown (n = 3 mice per group). Mean fluorescence intensities (MFI) of DHE staining were measured with image analysis software. Data are means ± SEM (n = 3 mice per group; ## p < 0.01; Cont. vs A/A Hep. b and (c) Immunohistochemistry of nitrotyrosine (b) and 4-HNE (c) in liver sections from 13-month-old Cont. and A/A Hep mice fed an HFrD for 5 wks, respectively. Representative images are shown (n = 4 ~ 5 mice per group). Inset shows a magnified view of the area outlined in the black dotted box. Quantification of nitrotyrosine and 4-HNE-positive cells is shown in the graphs. Data are means ± SEM (n = 4 ~ 5 mice per group); ## p < 0.01 and ### p < 0.001; Cont. vs A/A Hep

Back to article page