Fig. 2From: Recombinant SFRP5 protein significantly alleviated intrahepatic inflammation of nonalcoholic steatohepatitisIdentification and in vitro activity test of recombinant SFRP5. a SDS-PAGE of purified SFRP5 protein. 1. Purified SFRP5 (reductive with DTT), M: Marker (130kd,100kd,70kd,55kd,35kd,25kd,15kd,10kd). 2. Purified SFRP5 (non-reductive) (b) western blot of purified SFRP5 protein. 1. Purified SFRP5 (reductive with DTT), 2. Purified SFRP5 (non-reductive), M: Marker (130kd,100kd,70kd,55kd,35kd,25kd,15kd,10kd). c HPLC of purified SFRP5 protein. d Principle of the binding test of SFRP5 with Wnt-5a. e The binding curve shows SFRP5 combines with Wnt-5a specifically in vitroBack to article page