Fig. 2

AA2P promotes the adipogenesis of 3T3-L1 cells. Oil red O staining, RNA and protein extraction were performed at D0, D3, D7 and D14 after non-induction or induction. AA2P was added to induce the 3T3-L1 preadipocytes on the overall stage of adipogenesis. a Oil red O staining; b mRNA levels of collagen VI and adipogenic-specific genes in the 3T3-L1 cells detected by qPCR. mRNA levels of the target gene are normalized to β-actin. Values are expressed as the mean ± SEM. *P < 0.05, **P < 0.01 versus the control group (n = 3); c Western blotting results at the same stage with mRNA levels; d Relative expression of the target protein