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Fig. 3 | Nutrition & Metabolism

Fig. 3

From: Ascorbic acid promotes 3T3-L1 cells adipogenesis by attenuating ERK signaling to upregulate the collagen VI

Fig. 3

Inhibition of collagen affects the adipogenesis of the 3T3-L1 cells. Oil red O staining, RNA and protein extractions were performed at D0, D3, D7 and D14 after induction. EDHB was added at the overall stage of adipogenesis or after treating with AA2P. a Oil red O staining; b mRNA levels of collagen VI and adipogenic-specific genes in the 3T3-L1 cells detected by qPCR. mRNA levels of the target gene are normalized to β-actin. Values are expressed as the mean ± SEM. *P < 0.05, **P < 0.01 versus the control group (n = 3); c Western blotting results at the same stage with mRNA levels; d Relative expression of the target protein

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Nutrition & Metabolism

ISSN: 1743-7075

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