Table 1 The sequences of the gene-specific primers
Gene | GenBank No. | Primer | Length |
|---|---|---|---|
Collagen VI α1 | NM_009933 | F: 5’-TGCCCTGTGGATCTATTCTTCG-3′ R: 5’-CTGTCTCTCAGGTTGTCAATG-3′ | 107 bp |
SREBP-1c | NM_011480 | F: 5’-GTGAGCCTGACAAGCAATCA-3′ R: 5’-GGTGCCTACAGAGCAAGAG-3’ | 102 bp |
C/EBPβ | NM_009883 | F: 5’-ACACGGGACTGACGCAACAC-3′ R: 5’-AACCCCGCAGGAACATCTTT-3’ | 78 bp |
PPARγ2 | NM_011146 | F: 5’-GTCATCCTGCTCTTCTTTCTCG-3′ R: 5’-ATGGCGTCCCTTCTCCTGT-3’ | 115 bp |
C/EBPα | NM_007678 | F: 5’-CAGGAGGAAGATACAGGAAGC-3′ R: 5’-TCTCCATGAACTCACCCAGG-3’ | 229 bp |
β-actin | NM_007393 | F: 5’-CCTCTATGCCAACACAGTGC-3′ R: 5’-GTACTCCTGCTTGCTGATCC-3’ | 193 bp |