Fig. 1

Detection of protein-protein interaction by acceptor bleaching method. HEK293T cells were co-transfected with Sel F’-pEYFP-N1 and pECFP-C1-RDH11 for sample tests (a). Then, cells were co-transfected with pEYFP-N1 and pECFP-C1-RDH11 (b) with ECFP-C1 and SEL F’-pEYFP-N1 (c) as negative controls. (1) Photobleaching curves (solid lines for donor fluorescence and dashed lines for receptor fluorescence). The region of interest (ROI) was bleached at 515 nm. (2) The fluorescence images of donors before bleaching. (3) The fluorescence images of donors after bleaching. (4) Donor fluorescence increments before and after bleaching. (5) Diagram of the distance between donor and receptor. (6) FRET efficiency diagram