Fig. 2

Verification of the interaction between SELENOF and RDH11 by Co-Immunoprecipitation (Co-IP) assay. HEK293T cells were co-transfected with plasmids RDH11-YFP-N1 and SelenoF-HA-SelExpress1 (or RDH11-YFP-N1 and HA-SelExpress1/SELENOF-HA-SelExpress1 and YFP-N1 as the negative controls) . The supernatants of the cell lysates were immunoprecipitated with an anti-HA antibody (a) or anti-GFP antibody (b) and analyzed by western blot (WB) using an anti-GFP antibody (a) or anti-HA antibody (b). The supernatant of the cell lysate, named Input, was used as a positive control for WB analysis and probed with anti-HA (b) or anti-GFP (a) antibody. IP Ex: IP extract