Fig. 3

Verification of the interaction between SELENOF’ and RDH11 by pull-downassay. SELENOF’-PET28b was transfermated into E.coli BL21 (DE3) and recombinant SELENOF’-his was purified by Ni-NTA; RDH11-EYFP-N1 was transfected into HEK293T celss. Cell lysates containing RDH11-EYFP-N1 and purified SelenoF’-His were incubated with Ni-NTA resin (pull-down) and then detected by anti-GFP antibody. The supernatant of the cell lysate containing RDH11-EYFP-N1, named Input, was used as a positive control for WB analysis probed with an anti-GFP antibody. Cell lysates containing the EYFP-N1 vector and purified SelenoF’-His were used as negative controls