Table 1 Primers used for plasmids construction
Plasmids constructed | Sequence | |
|---|---|---|
SEL F′-YFP-N1 | F | 5’-ATGGTAGCGATGGCGGCTGGGC-3′ |
R | 5’-TTATATGCGTTCCAACTTTTCAC-3′ | |
NpGBKT7-Sel F′ | F | 5’-TTTGGGGCAGAGTTTTCATCGGAG-3’ |
R | 5’-TTATATGCGTTCCAACTTTTCAC-3’ | |
RDH11-YFP-N1 | F | 5’-CGGAATTCATGGTTGAGCTCATGTTCCCGCTGT-3’ |
R | 5’-TAGGTACCCTGTCTATTGGGAGGCCCAGCAGGTC-3’ | |
RDH11-CFP-C1 | F | 5’-CGGAATTCCGTTGAGCTCATGTTCCCGCTGT-3’ |
R | 5’-TAGGTACCTGGGTCCAACTGGCACTGCCTGTTA-3’ | |
SEPT3-CFP-C1 | F | 5’-ATAAGCTTATTCCAAAGGGCTCCCAGAGAC-3’ |
R | 5’-TAGGTACCTCATGGGTTACTGTCGTGGCTTT-3’ | |
TUBB4-CFP-C1 | F | 5’-TAGAATTCACGGGAGATCGTGCACCTGCAGG-3’ |
R | 5’-TAGGTACCCTAGGCCACCTCCTCCTCCGCCT-3’ | |