Fig. 2

Fiber type composition of longissimus dorsi (a) and biceps femoris (b) muscle of pigs of the groups CON, CARN, PIV and CARN + PIV (experiment 2). Representative images were obtained from serial muscle cross-sections incubated with a primary antibody cocktail against MHCI (BA-F8), MHC IIb (BF-F3) and all MHC isoforms except MHC IIx (BF-35), followed by incubation with appropriate fluorescent-conjugated secondary antibodies. Images show the four major fiber types, type I (blue), type IIa (green), type IIb (red) and type IIx (unstained). Bars represent fiber type percentages obtained from fiber counting of immunohistochemically-stained muscle cross-sections at 100-fold magnification and are means ± SD of n = 12 piglets/group