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Table 1 IC50 values (in μM) for substances tested in LPS-activated RAW264.7 cells

From: Z-ligustilide and anti-inflammatory prostaglandins have common biological properties in macrophages and leukocytes

  PGE2   Nitric Oxide  
  Mean ± S.E.M. N Mean ± S.E.M. N
Z-ligustilide 9.3 ± 1.6 33 12.8 ± 1.4 58
15d–PGJ2 2.2 ± 0.5 16 2.3 ± 0.6 25
Rosiglitazone >50 2 26.9 ± 1.9 16
L-NAME >500 2 150 ± 12 2
  1. Cells (in triplicates) were incubated with graded amounts of substances, stimulated with 1 μg/mL LPS and cultured for 24 h. PGE2 and NO were measured by EIA and the Griess reaction, respectively, and the IC50 values were calculated for each experimental series. N: number of independent experimental series. L-NAME: L-NG-Nitroarginine methyl ester