Z-ligustilide and anti-inflammatory prostaglandins have common biological properties in macrophages and leukocytes

Table 3 Inflammatory proteins secreted by human PBLs

Protein	Unstimulated cells 24 h) [pg/mL]	Protein secretion (ratio LPS-stim./ unstim.)	% inhibition by Lig (6.25 μM)	% inhibition by 15d–PGJ ₂ (1.25 μM)
CCL2/MCP-1	8540	0.8	87 ± 3 ^**)	- 37 ± 34
CCL3/MIP-1α	6055	20.6	61 ± 4 ^*)	67 ± 36 ^**)
CCL4/MIP-1β	35,000	7.0	68 ± 7 ^**)	51 ± 14 ^*)
CCL5/RANTES	9225	0.8	< 2	<2
CXCL8/IL-8	20,700	21.1	<2	<2
CXCL10/IP-10	529	22.6	89 ± 3 ^**)	86 ± 12 ^**)
IFN-γ	22	10.4	84 ± 1 ^**)	85 ± 9 ^**)
TNF-α	982	24.8	46 ± 4 ^*)	−16 ± 18
IL-1β	1055	15.5	−57 ± 5 ^*)	38 ± 0 ^*)
IL-6	11,400	8.6	−51 ± 3 ^**)	−135 ± 32 ^**)
IL-12p70	12	21.1	89 ± 2 ^**)	85 ± 12 ^**)

Peripheral blood leukocytes were isolated and cultured for 24 h at the indicated treatments. The proteins secreted into the culture supernatants were measured by multiparametric analysis. * p < 0.05; ** p < 0.01; *** p < 0.005 (‘LPS + substance’ treated versus ‘LPS-only’ treated cells)

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