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Table 1 Addition of substrate and inhibitors

From: Associations between fatty acid oxidation, hepatic mitochondrial function, and plasma acylcarnitine levels in mice

Compound End concentration Purpose
Malate 0.5 mM Supplies complex I with substrate
Octanoylcarnitine 0.2 mM Supplies ETF with electrons
ADP 2.5 mM To assess oxidative phosphorylation
Glutamate 10 mM Supplies complex I with substrate
Succinate 10 mM Supplies complex II with substrates
FCCP   Uncouples ETS from oxidative phosphorylation
Rotenone 0.5 μM Inhibits the electron flux from the Fe-S centre to Q in complex I
Antimycin A 2.5 mM Inhibits the electron flux from heme bH to Q in complex III, to observe non-mitochondrial respiration.