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Fig. 2 | Nutrition & Metabolism

Fig. 2

From: Hsp90β knockdown in DIO mice reverses insulin resistance and improves glucose tolerance

Fig. 2

siRNA mediated knockdown of Hsp90ab1 is isoform specific in human skeletal muscle myotubes (HSMM). HSMM were transiently transfected with siRNA targeting Hsp90ab1 (siHsp90ab1) or negative control (NC) siRNA for 48 h prior to qPCR analysis of Hsp90ab1, Hsp90aa1, Hsp90b1, TRAP1 mRNA. siRNA knockdown of Hsp90ab1 was isoform specific as mRNA expression of other Hsp90 isoforms were not affected. Data represent mean + SEM of n = 3 independent experiments. **p < 0.01 vs. NC, Student’s t-test

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