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Fig. 4 | Nutrition & Metabolism

Fig. 4

From: Hsp90β knockdown in DIO mice reverses insulin resistance and improves glucose tolerance

Fig. 4

Hsp90ab1 siRNA knockdown in HSMM increases insulin signaling. HSMM were transiently transfected with either NC siRNA or Hsp90ab1 siRNA for 48 h, serum starved for 3 h, and stimulated with 0 or 100 nM insulin for 5 min. Phosphorylated-Akt was measured by ELISA, normalized by total protein, and plotted as fold change relative to NC. HSMM transfected with siHsp90ab1 show increased insulin-induced phosphorylation of Akt relative to NC. Data represent mean + SEM of n = 6 independent samples. ****p < 0.0001 vs. 0 of respective cell line. ++p < 0.01 vs NC

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