Fig. 4
From: Hsp90β knockdown in DIO mice reverses insulin resistance and improves glucose tolerance
Hsp90ab1 siRNA knockdown in HSMM increases insulin signaling. HSMM were transiently transfected with either NC siRNA or Hsp90ab1 siRNA for 48 h, serum starved for 3 h, and stimulated with 0 or 100 nM insulin for 5 min. Phosphorylated-Akt was measured by ELISA, normalized by total protein, and plotted as fold change relative to NC. HSMM transfected with siHsp90ab1 show increased insulin-induced phosphorylation of Akt relative to NC. Data represent mean + SEM of n = 6 independent samples. ****p < 0.0001 vs. 0 of respective cell line. ++p < 0.01 vs NC