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Fig. 6 | Nutrition & Metabolism

Fig. 6

From: Hsp90β knockdown in DIO mice reverses insulin resistance and improves glucose tolerance

Fig. 6

Hsp90ab1 knockdown alters expression of metabolic genes and decreases PDH catalytic subunit phosphorylation. a Quantitative PCR analysis of mRNA expression of metabolic enzymes for glucose and lipid metabolism in skeletal muscle of DIO mice after 4 weeks antisense oligonucleotide (ASO) treatment at 10μg/kg/day shows that PFKM, GYS1 and ACADL are significantly increased over control (*p < 0.05, **p < 0.01, n = 10 for NC and n = 9 for ASO). b Quantitative PCR analysis of adipose triglyceride lipase (ATGL) mRNA expression shows a significant increase in response to Hsp90ab1 knockdown (n = 10 for NC and n = 9 for ASO, ****p < 0.0001). c Quantitative PCR analysis of PDK4 mRNA expression shows a significant decrease in response to Hsp90ab1 knockdown (*p < 0.05, n = 10 for NC and n = 9 for ASO). d Representative Western blot analysis of total and phosphorylated PDH E1α, the catalytic subunit of PDH complex, in muscles of DIO mice treated with NC ASO or Hsp90ab1 ASO (n = 5 per group). Phosphorylation of PDH E1α was examined at serine 232 (S232), serine 293 (S293), and serine 300 (S300). e Quantification of immunoreactive bands relative to total protein loaded as indicated by Ponceau S stain shows S232 and S300 are reduced in response to Hsp90ab1 over control (*p < 0.05, **p < 0.01, **** p < 0.0001 vs. NC, n = 5)

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