Fig. 5

The LPD prevents renal injuries, the impairment of autophagy and the activation of mTORC1 in the kidneys of Wistar fatty rats. (a) Representative microphotographs of Masson’s trichrome (MT) staining of glomeruli (scale bar: 100 μm) and the tubulo-interstitial area (scale bar: 1 mm) after the 24-week dietary intervention. (b) Glomerular fibrotic score (n = 3) and (c) tubulo-interstitial fibrotic score (n = 3), assessed using MT staining after the 24-week dietary intervention. mRNA expression of Col3 (d), Kim-1 (e), Cd68 (f), Ccl12 (g), Tlr4 (h) and Il6 (i) in the renal cortex, adjusted to 18 s levels, after the 24-week dietary intervention (n = 6 each). (j) Representative photographs of immunoblotting for p62, β-actin, p-S6RP and S6RP in the renal cortex after the 24-week dietary intervention (n = 3). Quantitative ratios of p62 to β-actin (k) and p-S6RP to S6RP (l) (n = 3). Col3: type 3 collagen, Kim-1: kidney injury molecule-1, Ccl2: C-C motif chemokine ligand 2, Tlr4: Toll-like receptor 4, Il6: interleukin-6, p-S6RP: phospho-S6 ribosomal protein, S6RP: S6 ribosomal protein. The data are shown as the mean ± SD. * p < 0.05, **p < 0.01 and *** p < 0.001 for the indicated comparison. n.s.: not significant