Fig. 2

Dual immunolabeling of SIRT1/SIRT7 (a-e) and SIRT2/α-actin (f-j) in erectile tissue of rats from all experimental groups (n = 3/group). SIRT1 was identified in the nucleus and cytoplasm (green) mainly in the SMC of all the analyzed tissues (a-e) and SIRT7 is apparently more expressed in the endothelium (red) (a-e). No marked differences were seen among groups for SIRT1 and SIRT7 expression. SIRT2 expression was observed in cytosol mainly in SMC in all experimental groups, often co-localizing with α-actin labeling (f-j). The co-localization seems to be more intense in CC from rats of HF/ER/S/Ex group (j). C-control; HF-high-fat diet treated rats; HF/ER-high-fat diet treated rats under energy restriction for 6 months; HF/ER/S-high-fat diet treated rats under energy restriction and atorvastatin treatment for 6 months; HF/ER/S/Ex-high-fat diet treated rats under energy restriction, atorvastatin treatment and exercise for 6 months. VS- vascular space