Fig. 3

Dual immunolabeling of SIRT3/α-actin (a-e) and SIRT4/α-actin (f-j) in erectile tissue of rats from experimental groups (n = 3/group). Sirtuins were labeled in green and α-actin in red. Mitochondrial SIRT3 and 4 were detected in co-localization with α-actin in the CC of all experimental groups (a-j). C-control; HF-high-fat diet treated rats; HF/ER-high-fat diet treated rats under energy restriction for 6 months; HF/ER/S-high-fat diet treated rats under energy restriction and atorvastatin treatment for 6 months; HF/ER/S/Ex-high-fat diet treated rats under energy restriction, atorvastatin treatment and exercise for 6 months. VS- vascular space