Fig. 5

Semiquantification of protein expression levels of the eNOS, iNOS, ET-1, and SIRT2,3,4,6 and 7 by western blotting. Representative blots for each studied protein and representative Ponceau S staining for samples of each group are ilustrated (a). Graphs (b-i) represent the densitometric quantification of each band relatively to the respective lane after Ponceau S staining. C-control; HF-high-fat diet treated rats; HF/ER-high-fat diet treated rats under energy restriction for 6 months; HF/ER/S-high-fat diet treated rats under energy restriction and atorvastatin treatment for 6 months; HF/ER/S/Ex-high-fat diet treated rats under energy restriction, atorvastatin treatment and exercise for 6 months. Error bars represent standard error for the mean (n = 5/group). ^* p < 0.05 vs C, ^# p < 0.05 vs HF, ⁺ p < 0.05 vs HF/ER, ^$p < 0.05 vs HF/ER/S