Fig. 5

The mRNA and protein expression of I-BABP in Caco-2 cells (n = 5). Total RNA and total protein were extracted from Caco-2 cells, the mRNA transcription level was measured using real-time PCR analysis, and the protein expression level was measured using Western blot analysis. The housekeeping gene β-actin was used to normalize the expression levels, and Critical threshold (Ct) values were compared in a, while relative light density values were compared in c. ^aP < 0.05 versus CA group; ^bP < 0.05 versus C18:0 + CA; ^cP < 0.05 versus C18:1 + CA group; a. critical threshold; b. section of blots; c. grey-scale analysis