Fig. 4

Effect of PF-4708671 on lipid accumulation and expression of lipogenic genes in HepG2 cells. Cells were exposed to 6 μM PF-4708671, 144 μM PA, or 14 nM RAPA for different periods of time. a After treatment (a control for 24 h; b PF-4708671 for 24 h; c PA for 24 h; d PA + PF-4708671 for 24 h; e PA + PF-4708671 + RAPA for 24 h; f control for 48 h; g PF-4708671 for 48 h; h PA for 48 h; i PA + PF-4708671 for 48 h; and j PA + PF-4708671 + RAPA for 48 h), the cells were stained with Oil Red O. b The intracellular lipid content in each group was quantified. c Detection of TG levels. After treatment. d Western blotting was used to detect the protein expression levels, and (e) qRT-PCR was used to detect the relative mRNA expression levels of lipogenic genes. Data are presented as the means±SDs of three independent experiments, each of which was performed in triplicate (*P < 0.05 versus the control group; #P < 0.05 versus the model group)