Fig. 5

Effect of LXRα knockdown on HepG2 cells. a Detection of LXRα knockdown efficiency by Western blotting. b Oil Red O staining (a normal control; b siRNA-LXRα; c PA; d PA + siRNA-LXRα; and e negative control). c The intracellular lipid content in each group was quantified. d Detection of TG levels. After transient transfection of HepG2 cell with siRNA-LXRα in the absence or presence of PA. e Western blotting was used to detect the protein expression levels, and (f) qRT-PCR was used to detect the relative mRNA expression levels of lipogenic genes. Verification of the inhibitory effect of LXRα knockdown, RAPA, and PF-4708671, alone and in combination. Protein expression levels (g) and mRNA expression levels (h) were assessed by Western blotting and qRT-PCR, respectively. Data are presented as the means±SDs of three independent experiments, each of which was performed in triplicate (*P < 0.05 versus the control group; #P < 0.05 versus the model group)