GLUT1 and lactose synthetase are critical genes for lactose synthesis in lactating sows

Zhang, Yinzhi; Zhang, Shihai; Guan, Wutai; Chen, Fang; Cheng, Lin; Lv, Yantao; Chen, Jun

doi:10.1186/s12986-018-0276-9

Table 2 Primers used for RT-PCR

From: GLUT1 and lactose synthetase are critical genes for lactose synthesis in lactating sows

Gene name	Gene accession	Primer sequence (5′-3′)	Size (bp)
GLUT1	EU012358	F: GATGAAGGAGGAGTGCCG	106
GLUT1	EU012358	R: CAGCACCACGGCGATGAGGAT	106
SGLT1	NM001164021	CATCATCGTCCTGGTCGTC	138
SGLT1	NM001164021	GGGGCTTCTTGAATGTCCT	138
HK1	XM003359224	CGAGATAACAAGAGCACACCC	138
HK1	XM003359224	GAGGAAACGCACATCACAGTC	138
HK2	DQ432056	GTTCCTGGCTCTGGATCTTGG	115
HK2	DQ432056	GGGATGGCGTAGATCTGGTTC	115
LALBA	NM214360	GTGGTGGGGATTCTCTTTCC	179
LALBA	NM214360	TCTGTGCTGCCATTGTCATG	179
B4GALT1	XM003130680	GAGTTTAACATGGCCGTGGAC	185
B4GALT1	XM003130680	TGACGCTGTAGGATTGGGTG	185
PGM1	NM001246318	TGGTCGCTTGGTTATTGG	123
PGM1	NM001246318	TTGGGTCCTCCTGGGTTGT	123
PGM2	XM003128910	CAACAGTGACGCACCCGA	198
PGM2	XM003128910	GCCACCAGCCCAAGAGG	198
UGP2	NM213980	TACCACGGCACCATCACA	238
UGP2	NM213980	GGTTCCCAAACCACCATT	238
GALT	XM005668043	TGGTTGGCTACGAAATGC	126
GALT	XM005668043	TGGCTGCTGTCTCCTTGT	126
GALE	XM003356202	GACTATGACACGGAGGATGG	108
GALE	XM003356202	CGTGCCCAGGTTGTAGAT	108
SLC35A2	XM013986120	GCTGTGGTCATGGCTGAAGT	196
SLC35A2	XM013986120	TGGAGATGGCAACATACTGGA	196
AKT1	NM001159776	CCTGAAGAAGGAGGTCATCG	123
AKT1	NM001159776	TCGTGGGTCTGGAAGGAGTA	123
PRLR	NM001001868	TTTCTGCTGTCGTCTGTTTGA	142
PRLR	NM001001868	TCTTCGGACTTGCCTTTCTC	142
STAT5a	NM214290	CATCACCATTGCCTGGAAG	141
STAT5a	NM214290	CGGTCGGGAAACACATAGAT	141
STAT5b	NM214168	TGTGAGAAGTTGGCGGAGAT	169
STAT5b	NM214168	CGATGATGAATGTGCTGGTC	169
ACACA	NM001114269	ACATCCCCACGCTAAACA	186
ACACA	NM001114269	AGCCCATCACTTCATCAAAG	186
FASN	NM001099930	GCTTGTCCTGGGAAGAGTGTA	115
FASN	NM001099930	AGGAACTCGGACATAGCGG	115
PGLS	XM003123494	GCTGGACTCTGGGCTTCTG	138
PGLS	XM003123494	GCAGCTCAGGGTTAATGGTG	138
β-ACTIN	XM003124280	GGATGCAGAAGGAGATCACG	105
β-ACTIN	XM003124280	ATCTGCTGGAAGGTGGACAG	105
GAPDH	NM001206359	AAGGTCGGAGTGAACGGATT	248
GAPDH	NM001206359	CATTTGATGTTGGCGGGAT	248
MRPL39	AY610067	CAAAAGAGAACCTACATTCCTTCACA	100
MRPL39	AY610067	TCTAATGCCACTTTTGCTTCAACT	100
SDHA	DQ402993	ACTCGCTCCTGGACCTCGT	152
SDHA	DQ402993	GGTTCCGTTCGCAAATCTC	152
TBP	DQ178129	GATGGACGTTCGGTTTAGG	124
TBP	DQ178129	AGCAGCACAGTACGAGCAA	124

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