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Fig. 3 | Nutrition & Metabolism

Fig. 3

From: Novel anti-obesity effects of beer hops compound xanthohumol: role of AMPK signaling pathway

Fig. 3

XN increases mitochondrial content, oxygen consumption rate, and upregulates thermogenic marker expression in 3T3-L1 adipocytes. a. Various doses of xanthohumol (6.25 and 25 μM) were incubated with mature, 3T3-L1 adipocytes, followed by quantification of mitochondrial content using MitoTracker® Green FM assay. All data are presented as the mean ± SEM. Means denoted with different letters are statistically different, P < 0.05. b. Protein expression levels of PGC-1α, a regulator of mitochondrial metabolism in 3T3-L1 adipocytes after 24 h of treatment with XN or isoproterenol. c. UCP1 and TBX1 protein expression levels post-24 h XN or Iso stimulation. d. OCR as determined by the Oxygen Consumption Rate Assay Kit following 24 h of treatment with XN or isoproterenol. All data are presented as mean ± SEM. Statistical significance between control and treatment groups is depicted as *P < 0.05, **P < 0.01

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