Fig. 6

XN-induced inhibition of adipogenesis and stimulation of lipolysis is reversed in the presence of AMPK inhibitor, dorsomorphin. a 3T3-L1 preadipocytes were treated on day 0 until day 7 of maturation and lipid accumulation was quantified by AdipoRed™ assay. b Differentiated, mature adipocytes were stimulated with XN, dorsomorphin, or AICAR on day 10 for 72 h and Oil Red O staining was performed to measure lipid content. c Whole cell lysates were extracted and Western blotting experiments conducted to quantitate the expression of HSL (6c), ATGL (6c), and p-ACC (6c) protein levels post 1 h XN, dorso, or AICAR stimulation. All data are presented as the mean ± SEM. Means denoted with different letters are statistically different, P < 0.05