Fig. 3

Thermogenic capacity of iBAT. All data are means ± SEM and were collected from mice after 12-week feeding either control (C), HFD (HF), or n-3 long-chain polyunsaturated fatty acid (LCPUFA)-enriched HFD (HF/n-3). a Representative microscopic images of hematoxylin and eosin-stained paraffin sections of iBAT. Scale bars indicate 50 μm. b Western blot analyses of the mitochondrial proteins uncoupling protein 1 (UCP1; 32 kDa) and citrate synthase (CS; 52 kDa) in iBAT (n = 5–6). Shown are the protein expression levels relative to controls. HSP90 (90 kDa) was used for protein normalisation. c Relative capacity index of UCP1 and CS (normalised values were used, see Methods). d UCP1 content in total iBAT (normalised values were used, see Methods). e Gene expression levels of peroxisome proliferator-activated receptor γ coactivator 1-α (Pgc1α) and -β (Pgc1β), genes involved in mitochondrial biogenesis, and of the endothelial marker cadherin 5 (Cdh5) in iBAT (n = 9–12). RT-qPCR data were normalised to CypB and Hsp90αb1, and calculated relative to controls. *p < 0.05, **p < 0.01, ***p < 0.001, significant differences compared to control or between groups as indicated