Fig. 6
Inflammatory status of iBAT. All data are means ± SEM and were collected from mice after 12-week feeding either control (C), HFD (HF), or n-3 long-chain polyunsaturated fatty acid (LCPUFA)-enriched HFD (HF/n-3). a Gene expression data of macrophage markers in iBAT (n = 10–12): EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1; F4/80), integrin αX (ITGAX; CD11c), mannose receptor C type 1 (MRC1; CD206) and C-type lectin domain family 10, member A (CLEC10a; CD301). b, c Quantification of cluster of macrophages (MCL) and solitary cells, detected by immunohistochemical analyses (images provided in Fig. 7), which are positive for F4/80, CD11c, CD206 or CD301 in iBAT (n = 4–5). Number of (b) positive-stained MCL and (c) positive-stained solitary cells is expressed as MCL or solitary cells per mm2 of tissue section. Gene expression data of (d) pro- or anti-inflammatory chemokines / cytokines and (e) an eosinophil-specific marker in iBAT (n = 7–12). RT-qPCR data were normalised to CypB and Hsp90αb1, and calculated relative to controls. *p < 0.05, **p < 0.01, ***p < 0.001, significant differences compared to control or between groups as indicated