Dietary n-3 long-chain polyunsaturated fatty acids upregulate energy dissipating metabolic pathways conveying anti-obesogenic effects in mice

Table 1 Diet compositions

Nutrient	C	HF	HF/n-3
Lipids (%)	5.01	25.04	25.02
Soybean oil	5.00	5.00	5.00
Palm oil	–	20.00	11.25
EPAX 1050 TG (DHA/EPA)	–	–	8.75
Cholesterol	0.013	0.038	0.021
Carbohydrates (%)	63.39	43.39	43.39
Corn starch	47.79	27.79	27.79
Protein (%)	24.00	24.00	24.00
Antioxidants (%)	0.03	0.03	0.03
Others (%)	7.60	7.60	7.60
Metabolizable energy (MJ/kg)	15.50	19.80	19.80
Fat (kJ%)	13.00	48.00	48.00
Carbohydrates (kJ%)	64.00	34.00	34.00
Protein (kJ%)	23.00	18.00	18.00
Fatty acid composition (%)
Ʃ SFA	16.55	42.66	29.83
Ʃ MUFA	25.95	37.14	29.05
Ʃ PUFA	56.90	19.70	40.12
Ʃ n-6 PUFA	50.85	18.07	18.19
Ʃ n-3 PUFA	5.16	1.34	21.68
n-6/n-3 PUFA ratio	9.85	13.50	0.84
Metabolizable energy, fat and fatty acids (kJ%)	13	48	48
Ʃ SFA	2.2	20.5	14.3
Ʃ MUFA	3.4	17.8	14.0
Ʃ PUFA	3.6	9.5	19.3
Ʃ n-6 PUFA	3.2	8.7	8.7
Ʃ n-3 PUFA	0.3	0.6	10.4

Shown are weight% or energy% macronutrients and other ingredients in different diets and the fat content is further characterised. Nutrient composition was kept constant across different diets, except for corn starch and fat components. Metabolizable energy was calculated according to Atwater in MJ/kg (provided by Ssniff Spezialdiäten) C control diet (Cat. no.: S5745-E720), DHA docosahexaenoic acid, EPA eicosapentaenoic acid, HF high-fat diet (Cat. no.: S5745-E722), HF/n-3 n-3 long-chain polyunsaturated fatty acid-enriched high-fat diet (Cat. no.: S5745-E725), MUFA monounsaturated fatty acids, PUFA polyunsaturated fatty acids, SFA saturated fatty acids

ISSN: 1743-7075